Would you expect the dissolved oxygen levels in water sampled from a lake at 7am to be higher or lower than the dissolved oxygen levels in water sampled at 5pm. After conducting this lab it was determined that primary productivity can indeed be measured using dissolved oxygen levels. Distilled water contains no organisms, is stagnant, and is kept at room temperature. The faster the water moves, the more dissolved oxygen the water will contain since it has more contact time with the air. By engaging with these educational materials, students learn.
You can save time by assigning each group one temperature of water for do determination and taking class averages. The light oxygen has 16 mg dissolved oxygen and then subtracted to 6 mg dissolved oxygen so that equals to 10 mg dissolved oxygen by net productivity. The neosci dissolved oxygen and aquatic primary productivity neo lab ap biology is computerbased interactive exercises on modeling pond ecosystem productivity including dissolved oxygen do and temperature and productivity as a function of depth in a lake for advanced placement ap biology students in grades 1112, and includes a narrated tutorial, assessments of student comprehension, and customized teacher resources. Dissolved oxygen and aquatic primary productivity kit. From this lab i have learned that the amount of dissolved oxygen created varies on the ecosystem itself and the factors in the ecosystems that effect the dissolved oxygen level. It goes on for several pages so i am only paraphrasing. View lab report lab 21 dissolved oxygen and aquatic primary productive from bio 101 at bremen high school, bremen.
Biondo september 19, 2012 design diagram dissolved oxygen. All of these physical factors, along with oxygen concentration, work together to increase diversity in aquatic habitats. Ap bio lab 12 dissolved oxygen college confidential. Andersen demonstrates the azidewinkler method of dissolved oxygen. The dissolved oxygen probe can be used for doing the do lab, but this is a very expensive probe. However, more oxygen is mixed into the water with the help of winds, rain, waves, and currents. Now, in your own words, explain why this calculation works. Measurements of dissolved oxygen do are used to determine primary productivity in bodies of water. Some of the factors that affect the amount of oxygen dissolved in water are. Videos anatomy and physiology ap biology ap chemistry. How would the predicted levels of do differ in each of the following pairs of water samples. To determine if water temperature affects dissolved oxygen concentration.
Dissolved oxygen is necessary for the metabolic processes of almost every organism. Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the classic edition of the biology place to all educators and their students. Measure the amount of dissolved oxygen in water samples at different temperatures 2. Includes instructions on water sampling, an illustrated.
Oxygen is essential for cellular respiration in most organisms. Andersen demonstrates the azidewinkler method of dissolved oxygen analysis. Effect of temperature on dissolved oxygen we actually did this part of the lab already. Analysis of dissolved oxygen lab have faith in ap biology. The advanced placement biology course is the equivalent of a twosemester college introductory biology course taken by biology majors during their first year. Neosci dissolved oxygen and aquatic primary productivity. Always wash hands thoroughly with soap and water after handling reagents or bio.
I found the discussion of dissolved oxygen in one of my old limnology books to be quite helpful. Create a hot water bath by performing the following steps. Who has answers for the questions from the ap bio college board lab 12. Determine if you will use a stovetop or microwave to heat the water. In this exercise you will be doing several calculations which analyze the dissolved oxygen concentration in. Neosci dissolved oxygen and aquatic primary productivity neo. Andersen demonstrates the azide winkler method of dissolved oxygen analysis. Dissolved oxygen and aquatic primary productivity lab. The 8station kit includes enough materials for a class of 32 students working in groups of 4. Since 1989, there have been 12 laboratorieslovingly nicknamed the dirty dozenthat are suggested curricula for ap biology classes. To be successful, a method for measuring dissolved oxygen must meet two requirements. Explain the relationship of dissolved oxygen to primary productivity. Remove the do probe from the storage bottle and carefully insert it into the culture.
We also predict that all of our experimental variables will contain more dissolved oxygen than our control, the distilled water. I compared it to a chart of 100 percent oxygen solubility at different temperatures, and in all cases for the chart values at 100 percent, the nomograph reads. Lab 21 dissolved oxygen and aquatic primary productive ap. They span the globe in their topics and their techniques. In this exercise you will be doing several calculations which analyze the dissolved oxygen concentration in water samples. Dissolved oxygen levels are an extremely important factor in determining the quality of an aquatic environment. Dissolved oxygen levels are an extremely important factor in determining the.
Ap biology dissolved oxygen and aquatic primary productivity purpose. Chemicals for the winkler technique are provided in groupsized increments. Dissolved oxygen and primary productivity 6 for evaluation purposes only 39. Dissolved oxygenclassic lab kit for ap biology, 3 groups. In the dissolved oxygen classic lab kit for ap biology, the relationship between the amount of do in water and water quality are explored and quantified. The neosci dissolved oxygen and aquatic primary productivity neolab ap biology is computerbased interactive exercises on modeling pond ecosystem productivity including dissolved oxygen do and temperature and productivity as a function of depth in a lake for advanced placement ap biology students in grades 1112, and includes a narrated tutorial, assessments of student comprehension. After showing themselves to be qualified on the ap exam, some students, as college freshmen, are permitted to take upperlevel biology courses or register for courses for which biology is. Dissolved oxygen prentice hall ap bio lab 12 lab bench quiz. While i was recently going over the dissolved oxygen data from lab 12, i realized that the nomograph in edition d page 140 of the ap lab manual is very poor and inaccurate. File type pdf ap biology lab 11 answers ap biology lab 11 answers ap biology lab 11. The silver ring on the probe should be immersed in the culture, but the probe should not. Remind yourself of our experiment, our procedures, and our conclusions. The test results indicated that after a 24hour time period, there was a difference in the primary productivity relative the amount of light tested. Andersen walks you through the cellular respiration lab.
These laboratory materials have been prepared by edvotek, inc. Oxygen is used by organisms in aerobic respiration, where energy is. September 2009 edited september 2009 in ap tests preparation. Usgs scientist is measuring various waterquality conditions in holes creek at huffman park in kettering, ohio. Lab 21 dissolved oxygen and aquatic primary productive. Print out the completed calculation table from sample problem page of the labbench web site. Definitely order the colorimeter, which can easily be used for doing the photosynthesis lab. Videos anatomy and physiology ap biology ap chemistry ap environmental science ap physics biology chemistry earth. Oxygen is required in cellular respiration, so it is very important for aquatic life. These all contribute to a lower dissolved oxygen concentration.
Dissolved oxygen lesson plan template and teaching resources. Safe and compact titration assemblies providedselect from an extensive catalog of educational tools available for enhancing the teaching of biological subjects, including simulated testing of blood and urine, the illustration of various natural habitats, and the dissection of sterilized stool samples collected from different regions. An individual license is valid for a single installation. I now have a better understanding of why ecosystems create and or need dissolved oxygen and the whole process of primary productivity. The amount of dissolved oxygen in a stream or lake can tell us a lot about its water quality. The concentration of dissolved oxygen in water depends also on temperature, pressure, and concentrations of various ions cf. The following citation format will be appropriate for lab reports based on one of the 12 ap biology labs. We were investigating the effect of the physical factor of temperature on the percent dissolved oxygen in a body of water.
At 4,000 meters in elevation,000 feet, the amount of dissolved oxygen in water is less than twothirds what it is at sea level. Terrestrial environments hold over 95% more oxygen than aquatic environments. The 1station kit includes materials for 1 group of 4 students. To explore, find and select your class from the tabs above. Seeing that the results were consistent through multiple experiments, it is highly likely that if the experiment was repeated. Dissolved oxygen and aquatic primary productivity introduction in an aquatic environment, oxygen must be dissolved in order to be available for use by organisms. The neosci dissolved oxygen and aquatic primary productivity neolab ap biology is computerbased interactive exercises on modeling pond ecosystem productivity including dissolved oxygen do and temperature and productivity as a function of depth in a lake for advanced placement ap biology students in grades 1112, and includes a narrated tutorial, assessments of student comprehension, and. Since oxygen is one of the most easily measured products of both photosynthesis and respiration, a good way to gauge primary productivity in an aquatic ecosystem is to measure dissolved oxygen. In conclusion, an increase in temperature and an increase in light increase the amount of dissolved oxygen within a water sample. Questions the discussion questions found throughout the laboratory should be written and answered in. You have 5 minutes to write in your lab notebook what you understand about the element oxygen and its role in aquatic biology.
We cannot measure gross productivity directly because respiration, which uses up oxygen and organic compounds, is always occurring simultaneously with. The silver ring on the probe should be immersed in the culture, but the probe should not be touching the bottom of the beaker. Temperature and dissolved oxygen the advanced placement biology laboratory manual recommends that each student group determine do of water samples at three different temperatures. Measure the amount of dissolved oxygen in water samples at different temperatures lab 12. Dissolved oxygen do is a measure of how much oxygen is dissolved in the water the amount of oxygen available to living aquatic organisms. The difference in the dissolved oxygen levels between the uncovered tube and the covered tube is the biochemical oxygen demand of the water sample. The advanced placement biology laboratory manual recommends that each student group determine do of. To measure and analyze the primary productivity of natural waters of laboratory cultures using screens to simulate the decrease of light with increasing depth. Dissolved oxygen and aquatic primary productivity, answer the following questions before midnight the day before the lab or you can not do the lab. Ap biology lab manual for teachers supplement technology integration use of gas pressure probeware provides a different means of running lab 2 a more rapidly, b without the necessity of using caustic sulfuric acid or messy permanganate stain and c without timing problems involved in stopping the reaction at specific points. The neosci ap biology neolab software series is compatible with microsoft windows 2000, windows xp, or windows vista, and mac os 9. The advanced placement ap program is a registered trademark of the college. Animal behavior paul andersen introduces the concept of ethology and contrasts kinesis and taxis.
In an aquatic environment, oxygen availability is influenced by a variety of chemical and physical factors. Dissolved oxygen levels are used to monitor the health and productivity. The instructor information section included for each experiment contains tips for successful completion, reagent preparation information, and. Dissolved oxygen and primary aquatic productivity laboratory 12 introduction dissolved oxygen levels are an extremely important factor in determining the quality of an aquatic environment. Dissolved oxygen and aquatic primary productivity by. Ap biology lab manual for teachers supplement when burettes are not available, titration can be done with droppers counting drops or with syringes which can easily measure mls. Ap sample lab 12 dissolved oxygen biology junction. The effect of the temperature of water on the concentration of dissolved oxygen hypothesis. In the dissolved oxygen classic lab kits for ap biology, the relationship between the amount of do in water and water quality are explored and quantified using the winkler method. The relationship between temperature, do, and respiration rates although water is composed of oxygen and hydrogen atoms, biological life in water depends upon another form of oxygenmolecular oxygen. Dissolved oxygen and primary productivity lab savage science. Biology with vernier addresses the fundamentals of a high school or college general biology course with 31 experiments that include cell respiration, photosynthesis, membrane diffusion, osmosis, human physiology, transpiration, fermentation, and more. Oxygen from the atmosphere is mixed into the water through diffusion.
Dissolved oxygen and primary aquatic productivity laboratory 12. We were investigating the effect of the physical factor of. Differences in temperature will not show differences in dissolved oxygen concentration. If the temperature of the water is increased, then the concentration o.
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